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  • ASTM
    E3273-21 Standard Practice to Assess Microbial Decontamination of Indoor Air using an Aerobiology Chamber
    Edition: 2021
    $78.62
    Unlimited Users per year

Description of ASTM-E3273 2021

ASTM E3273-21

Active Standard: Standard Practice to Assess Microbial Decontamination of Indoor Air using an Aerobiology Chamber




ASTM E3273

Scope

1.1 This practice is to assess technologies for microbial decontamination of indoor air using a sealed, room-sized chamber (~24 m3) as recommended by the U.S. Environmental Protection Agency (3). The test microbe is aerosolized inside the chamber where a fan uniformly mixes the aerosols and keeps them airborne. Samples of the air are collected and assayed, firstly to determine the rates of physical and biological decay of the test microbe, and then to assess the air decontaminating activity of the technology under test as log10 or percentage reductions in viability per m3 (1). The air temperature and relative humidity (RH) in the chamber are measured and recorded during each test.

1.2 The chamber can be used to assess microbial survival in indoor air as well as to test the ability of physical (for example, ultraviolet light) and chemical agents (for example, vaporized hydrogen peroxide) to inactivate representative pathogens or their surrogates in indoor air.

1.3 This practice does not cover testing of microbial contamination introduced into the chamber as a dry powder.

1.4 This practice does not cover work with human pathogenic viruses, which require additional safety and technical considerations.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


Keywords

Acinetobacter baumannii; aerobiology; aerosols; decontamination; Klebsiella pneumoniae; indoor air; nebulization; Pseudomonas aeruginosa; soil load; Staphylococcus aureus; surrogate;


ICS Code

ICS Number Code 07.100.01 (Microbiology in general)


DOI: 10.1520/E3273-21

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About ASTM

ASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide.
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