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E2720-10 Standard Test Method for Evaluation of Effectiveness of Decontamination Procedures for Air-Permeable Materials when Challenged with Biological Aerosols Containing Human Pathogenic Viruses >
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E2720-10 Standard Test Method for Evaluation of Effectiveness of Decontamination Procedures for Air-Permeable Materials when Challenged with Biological Aerosols Containing Human Pathogenic Viruses, 2010
- Scope
- Referenced Documents
- Terminology
- Summary of Test Method
- Significance and Use
- Apparatus
- Reagents and Materials
- TABLE 1
- Sampling, Test Specimens, and Test Units
- Experimental Design
- Test Procedure
- Calculation or Interpretation of Results
- Report
- Precision and Bias
- Keywords
- X1. EXAMPLE DEVICE: OPERATION OF THE LABORATORY-SCALE AEROSOL TUNNEL (LSAT)
- X1.1 Diagram of the Laboratory-Scale Aerosol Tunnel (LSAT)
- FIG. X1.1
- X1.2 Parameters of the LSAT
- X1.3 Test Procedure
- X2. EXAMPLE DEVICE: THE AEROSOL CHAMBER TESTING SYSTEM
- X2.1 Diagram of the Aerosol Chamber Testing System
- X2.2 Test Parameters of the Aerosol Chamber Testing System
- X2.3 Test Procedure
- FIG. X2.1
- X3. TCID50 ASSAY FOR INFLUENZA
- X3.1 Reagents
- X3.2 Method
- X4. EXAMPLE CALCULATION FOR TISSUE CULTURE INFECTIOUS DOSE (TCID50) ASSAY
- X4.1 24-Well Plate
- X4.2 96-Well Plate
- FIG. X4.1
- FIG. X4.2
- X5. EXAMPLE CALCULATIONS FOR DATA QUALITY OBJECTIVES AND DECONTAMINATION EFFICIENCY
- X5.1 Example Data Set
- X5.2 Average PFU per cm2
- X5.3 Data Quality Objectives
- X5.4 Decontamination Efficiency
- X6. METHOD ADAPTATION FOR USING BACTERIOPHAGE MS2
- X6.1 Scope
- X6.2 Referenced Documents
- X6.3 Terminology
- X6.4 Summary of Test Tethod
- X6.5 Significance and Use
- X6.6 Additional Equipment
- X6.7 Additional Reagents and Materials
- X6.8 Sampling, Test Specimens, and Test Units
- X6.9 Experimental Design
- X6.10 Test Procedure
- X6.11 Calculation and Interpretation of Results
- X6.12 Additional Reporting
- X6.13 Precision and Bias
- X6.14 Additional Keywords
- X7. MS2 PLAQUE ASSAY
- X7.1 Preparation of E. coli (Day 1)
- X7.2 Inoculation of TSB With E. coli (Day 2)
- X7.3 Media Preparation and Plating (Day 3)
- X7.4 Prepare MS2 Soft-Agar Media
- X7.5 Plating of Samples [Go to Page]
