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eLibrary > 22/30460212 DC BS EN 16615. Chemical disinfectants and antiseptics. Quantitative test method for the evaluation of bactericidal and yeasticidal and/or fungicidal and/or tuberculocidal and/or mycobactericidal activity on non-porous surfaces with mechanical >

22/30460212 DC BS EN 16615. Chemical disinfectants and antiseptics. Quantitative test method for the evaluation of bactericidal and yeasticidal and/or fungicidal and/or tuberculocidal and/or mycobactericidal activity on non-porous surfaces with mechanical, 2022

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22/30460212 DC BS EN 16615. Chemical disinfectants and antiseptics. Quantitative test method for the evaluation of bactericidal and yeasticidal and/or fungicidal and/or tuberculocidal and/or mycobactericidal activity on non-porous surfaces with mechanical, 2022

41_e_stf (1).pdf [Go to Page]
1 Scope
2 Normative references
3 Terms and definitions
4 Requirements
5 Test methods [Go to Page]
5.1 Principle
5.2 Materials and reagents [Go to Page]
5.2.1 Test organism
5.2.2 Culture media and reagents [Go to Page]
5.2.2.1 General
5.2.2.2 Water
5.2.2.3 Medium
5.2.2.4 Diluent
5.2.2.5 Neutralizer
5.2.2.6 Sterile defibrinated sheep blood
5.2.2.7 Hard water for dilution of products
5.2.2.8 Interfering substances
5.3 Apparatus and glassware
5.4 Preparation of test organism suspensions and product test solutions [Go to Page]
5.4.1 Test organism suspensions [Go to Page]
5.4.1.1 General
5.4.1.2 Preservation and stock cultures of test organisms
5.4.1.3 Working culture of test organisms
5.4.1.4 Test suspension (N)
5.4.1.5 Validation suspension (NV)
5.4.1.6 Incubation and counting of the test suspensions
5.4.2 Product test solution
5.5 Procedure for assessing the bactericidal and yeasticidal and/or fungicidal and/or tuberculocidal and/or mycobactericidal activity of the product [Go to Page]
5.5.1 General [Go to Page]
5.5.1.1 Experimental conditions
5.5.1.2 Selection of neutralizer
5.5.1.3 General instruction for validation and control procedures
5.5.1.4 Equilibration of temperature
5.5.1.5 Precautions for manipulation of test organisms
5.5.1.6 Inoculation of the test-surface
5.5.2 Method [Go to Page]
5.5.2.1 General
5.5.2.2 Test Na (Determination of bactericidal and yeasticidal and/or fungicidal and/or tuberculocidal and/or mycobacericidal concentrations), water control NW
5.5.2.3 Neutralizer control B – verification of the absence of toxicity of the neutralizer
5.5.2.4 Method validation C – dilution-neutralization validation
5.5.2.5 Incubation and counting of the test mixture
5.6 Experimental data and calculation [Go to Page]
5.6.1 Explanation of terms and abbreviations [Go to Page]
5.6.1.1 Overview of the different suspensions and test mixtures
5.6.1.2 Vc-values
5.6.2 Calculation [Go to Page]
5.6.2.1 General
5.6.2.2 Determination of Vc-values
5.6.2.3 Calculation of N
5.6.2.4 Calculation of Na, NW, Dc0, Dct
5.6.2.5 Calculation of NV and NV0
5.6.2.6 Calculation of B and C
5.7 Verification of methodology [Go to Page]
5.7.1 General
5.7.2 Control of weighted mean counts
5.7.3 Basic limits
5.8 Expression of results and precision [Go to Page]
5.8.1 Overview of the different suspensions/test mixtures
5.8.2 Vc-values
5.8.3 Limiting test organism and bactericidal and yeasticidal concentration [Go to Page]
5.8.3.1 Bactericidal concentration
5.8.3.2 Yeasticidal concentration
5.8.3.3 Tuberculocidal concentration
5.8.3.4 Mycobactericidal concentration
5.8.4 Precision, repetitions
5.9 Interpretation of results – conclusion
5.10 Test report
Annex A (informative)Referenced strains in national collections
Annex B (informative)Neutralizers
Annex C (informative)Graphical representations of the test method
Annex D (informative)Example of a typical test report
Annex E (informative)Alternative drying endpoint
Annex F (informative)Additional test temperature [Go to Page]

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22/30460212 DC BS EN 16615. Chemical disinfectants and antiseptics. Quantitative test method for the evaluation of bactericidal and yeasticidal and/or fungicidal and/or tuberculocidal and/or mycobactericidal activity on non-porous surfaces with mechanical, 2022

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please select your company type below: