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eLibrary > BS EN 15634-4:2023 Foodstuffs. Detection of food allergens by molecular biological methods - Peanut (Arachis hypogaea). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR >

BS EN 15634-4:2023 Foodstuffs. Detection of food allergens by molecular biological methods - Peanut (Arachis hypogaea). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR, 2023

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BS EN 15634-4:2023 Foodstuffs. Detection of food allergens by molecular biological methods - Peanut (Arachis hypogaea). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR, 2023

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1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents [Go to Page]
5.1 General
5.2 Extraction reagents
5.3 DNA purification by means of solid phase extraction
5.4 Real-time PCR reagents
6 Apparatus and equipment [Go to Page]
6.1 General
6.2 DNA extraction
6.3 PCR
7 Procedure [Go to Page]
7.1 General
7.2 Sample preparation
7.3 Preparation of extracts [Go to Page]
7.3.1 DNA extraction with CTAB and DNA purification
7.3.2 Quantification of DNA concentration and purity/quality check
7.4 Real-time PCR set-up [Go to Page]
7.4.1 Reaction mix for real-time PCR
7.4.2 Positive control for DNA targets
7.4.3 Negative control for DNA targets
7.4.4 PCR inhibition control
7.4.5 Amplification reagent control
7.4.6 Extraction blank control
7.4.7 Positive extraction control
7.4.8 Temperature/time programme (real-time PCR)
7.4.9 Accept/Reject criteria
7.4.10 Identification
8 Validation [Go to Page]
8.1 General
8.2 Specificity
8.3 Ring trial validation study [Go to Page]
8.3.1 Setup of the ring trial study
8.3.2 Ring trial validation results [Go to Page]
8.3.2.1 Ring trial validation data
8.3.2.2 Robustness of the method
8.3.2.3 Limit of detection
9 Test report [Go to Page]

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BS EN 15634-4:2023 Foodstuffs. Detection of food allergens by molecular biological methods - Peanut (Arachis hypogaea). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR, 2023

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please select your company type below: