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BS EN ISO 10272-1:2017+A1:2023 Microbiology of the food chain. Horizontal method for detection and enumeration of Campylobacter spp - Detection method. Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp, >
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BS EN ISO 10272-1:2017+A1:2023 Microbiology of the food chain. Horizontal method for detection and enumeration of Campylobacter spp - Detection method. Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp,, 2023
- undefined
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 4.1 General
- 4.2 Enrichment in selective liquid medium
- 4.2.1 Detection procedure A
- 4.2.2 Detection procedure B
- 4.2.3 Detection procedure C
- 4.3 Isolation on selective solid medium
- 4.3.1 Detection procedure A
- 4.3.2 Detection procedure B
- 4.3.3 Detection procedure C
- 4.3.4 Detection procedure A, B and C
- 4.4 Confirmation
- 5 Culture media and reagents
- 6 Equipment and consumables
- 7 Sampling
- 8 Preparation of test sample
- 9 Procedure
- 9.1 General
- 9.2 Test portion and initial suspension
- 9.2.1 General
- 9.2.2 Detection procedure A
- 9.2.3 Detection procedure B
- 9.2.4 Detection procedure C
- 9.3 Enrichment
- 9.3.1 Detection procedure A
- 9.3.2 Detection procedure B
- 9.4 Isolation
- 9.4.1 Detection procedure A
- 9.4.2 Detection procedure B
- 9.4.3 Detection procedures A, B and C
- 9.5 Confirmation of Campylobacter
- 9.5.1 General
- 9.5.2 Selection of colonies for confirmation
- 9.5.3 Examination of morphology and motility
- 9.5.4 Study of aerobic growth at 25 °C
- 9.5.5 Detection of oxidase activity
- 9.5.6 Interpretation
- 9.6 Identification of Campylobacter species (optional)
- 9.6.1 General
- 9.6.2 Detection of catalase activity
- 9.6.3 Detection of hippurate hydrolysis
- 9.6.4 Detection of indoxyl acetate hydrolysis
- 9.6.5 Interpretation
- 10 Expression of results
- 11 Performance characteristics of the method
- 11.1 Interlaboratory study
- 11.2 Sensitivity
- 11.3 Specificity
- 11.4 LOD50
- 12 Test report
- Annex A (normative) Diagram of procedures
- Annex B (normative) Culture media and reagents
- Annex C (informative) Method validation studies and performance characteristics
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- Bibliography [Go to Page]