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BS EN ISO 22717:2015+A1:2022 Cosmetics. Microbiology. Detection of Pseudomonas aeruginosa, 2022
- undefined [Go to Page]
- Blank Page
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 5 Diluents and culture media
- 5.1 General
- 5.2 Diluent for the bacterial suspension (tryptone sodium chloride solution)
- 5.2.1 General
- 5.2.2 Composition
- 5.2.3 Preparation
- 5.3 Culture media
- 5.3.1 General
- 5.3.2 Agar medium for the suitability test (see Clause 11) [soybean–casein digest agar medium (SCDA) or tryptic soy agar (TSA)]
- 5.3.3 Enrichment broth
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- 5.3.4 Selective agar medium for isolation of Pseudomonas aeruginosa
- 5.3.5 Selective agar medium for confirmation of Pseudomonas aeruginosa
- 6 Apparatus and glassware
- 7 Strains of microorganisms
- 8 Handling of cosmetic products and laboratory samples
- 9 Procedure
- 9.1 General recommendation
- 9.2 Preparation of the initial suspension in the enrichment broth
- 9.2.1 General
- 9.2.2 Water-miscible products
- 9.2.3 Water-immiscible products
- 9.2.4 Filterable products
- 9.3 Incubation of the inoculated enrichment broth
- 9.4 Detection and Identification of Pseudomonas aeruginosa
- 9.4.1 Isolation
- 9.4.2 Identification of Pseudomonas aeruginosa
- 10 Expression of results (detection of Pseudomonas aeruginosa)
- 11 Neutralization of the antimicrobial properties of the product
- 11.1 General
- 11.2 Preparation of the inoculum
- 11.3 Suitability of the detection method
- 11.3.1 Procedure
- 11.3.2 Interpretation of suitability test results
- 12 Test report
- Annex A (informative) Other enrichment broths
- Annex B (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
- Bibliography [Go to Page]
