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eLibrary > BS ISO 18184:2019 - TC Tracked Changes. Textiles. Determination of antiviral activity of textile products >

BS ISO 18184:2019 - TC Tracked Changes. Textiles. Determination of antiviral activity of textile products, 2020

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BS ISO 18184:2019 - TC Tracked Changes. Textiles. Determination of antiviral activity of textile products, 2020

National foreword [Go to Page]
Compliance with a British Standard cannot confer immunity from legal obligations.
Amendments/corrigenda issued since publication
Textiles — Determination of antiviral
activity of textile products [Go to Page]
COPYRIGHT PROTECTED DOCUMENT
Foreword
Introduction
Textiles — Determination of antiviral activity of textile products [Go to Page]
1 Scope
2 Normative references
3 Terms and definitions [Go to Page]
3.1
3.2
3.3
3.4
3.5
reference cloth control fabric [Go to Page]
3.6
3.7
3.8
3.9
3.10
3.11
3.12
3.13
3.14
4 Principle
5 Virus and host cell
6 Warning
7 Apparatus [Go to Page]
7.6 Washing machine.
Figure 1 — 96 wells microplate for TCID50 method
Figure 2 — 6 wells plastic plate for plaque assay
Figure 3 — Flask for cell culture use
7.26 Centrifuge tube.
8 Sterilization of apparatus
9 Reagent and medium [Go to Page]
9.3 7,5 % sodium bicarbonate solution.
9.4 Formalin solution. [Go to Page]
9.5 Methylene blue solution, use for the cells dyeing.
9.4 Formaldehyde solution. [Go to Page]
9.5 Methylene blue solution.
9.6 Inactivated fetal bovine serum (FBS) [Go to Page]
9.7 Growth medium, used for cell culture.
9.8 Maintenance medium, used for cell culture.
9.9 Double concentration of the maintenance medium (9.8).
9.10 0,01 mol/l phosphate buffered saline PBS (-).
9.11 Trypsin derived from beef pancreas and PBS (-) solution.
9.12 Trypsin EDTA solution.
9.13 DEAE-dextran solution.
9.14 Agar medium, used for the plaque assay. preparation.
9.14.1 A liquid.Liquid A.
9.14.2 Liquid B liquid.
9.16 Maintenance medium, used for cell culture and for TCID50 method.Maintenance mediumwith Trypsin.
10 Preparation [Go to Page]
10.1 Restoration of host cell from cryopreservation
10.2 Subculture of host cell
10.3 Cell culture for the infectious virus titre assay
10.4 Preparation for test virus [Go to Page]
10.4.1 General
10.4.2 Influenza virus
10.4.3 Feline calicivirus
10.4.4 Infectivity titre of the test viruses
10.4.4.1 Preparation for series of the dilution for the virus suspension
10.4.4.12 Infectivity measurement
10.4.4.2 Determination of the infectious titre
10.5 Preparation for test specimen [Go to Page]
10.5.1 Control fabric
10.5.2 Preparation of test specimens
10.5.3 Sterilization of specimens
10.6 Control test [Go to Page]
10.6.1 General
10.6.2 Verification of cytotoxic effect
10.6.2 Verification of cytotoxicity by cell sensitivity to virus and the inactivation of antiviral activity
10.6.3 Verification of cell sensitivity to virus and the inactivation of antiviral activity [Go to Page]
11 Test procedure [Go to Page]
11.1 Preparation of specimen
11.2 Inoculation of virus to the specimens
11.2 Deposit of virus to the specimens
11.3 Contacting time
11.4 Wash-out of virus immediately after inoculationdeposit
11.5 Wash-out of virus after contacting time
12 Preparation of the series of the dilution for the virus suspension
13 Infective titre measurement
13 Determination of the infectious titre [Go to Page]
13.1 Plaque assay
14 Calculation of infectivity titre [Go to Page]
14.2.1 The Behrens and Karber method
14.2.2 Example of calculation
Figure 4 — Example of TCID50 method
14.3 Test result [Go to Page]
14.3.1 Verification of this test
14.3.2 Calculation of antiviral activity value
15 Test report
(normativeinformative) [Go to Page]
Table A.1 — Viruses, host cells and media used for this standarddocument
Annex B [Go to Page]
(normative) [Go to Page]
B.1 Test procedure [Go to Page]
B.2 Determination of PFU
B.2.1 General
Table B.2 — Interpretation of the data
Annex C [Go to Page]
(normative) [Go to Page]
C.1 Test procedure
C.1.1 Pick up 96 wells microplate with the monolayer cells grown in the each well of Clause 10.3 and observe by a microscope of a confluent state of the grown cells. After confirmation, drain extra growth medium from the plate.Observe at the microscop... [Go to Page]
D.2 Composition of EMEM [Go to Page]
Table D.1 — Composition of EMEM
Table D.2 — The Composition of RPMI 1640
Annex E [Go to Page]
(informative) [Go to Page]
E.1 General
Table E.1 — Polio virus strain and host cell [Go to Page]
F.1E.1 General
F.2 Scope
E.2 Overview
F.3.1 SPF embryonated hen's eggs
E.3 Specific pathogen free (SPF) embryonated hen's eggs
F.4E.4 Preparation of specimen
F.5E.5 Preparation of the embryonated hen's eggs
F.6E.6 Preparation of virus [Go to Page]
F.6.1E.6.1 Virus cultivation
F.6.2E.6.2 Preparation of 0,5 % chicken red blood cell suspension
F.7E.7 Test procedure [Go to Page]
F.7.1E.7.1 General [Go to Page]
Figure F.1Figure E.1— General process image of embryonated eggs method
F.7.3E.7.3 Inoculation of the reacted virus suspension to 10 day old embryonated hen's eggs
F.7.4E.7.4 Virus assay
F.7.5E.7.5 Calculation of the virus titre
Table F.1E.1 — Example of the EID test
Annex F [Go to Page]
(informative) [Go to Page]
Tabelle GTable F.1 — Antiviral performance standard
Table H.1 — Sample details for Round robin test
Table G.1 — Sample details for interlaboratory test
H.3G.3 Test condition
H.4G.4 Test result [Go to Page]
H.4.1G.4.1 Test result of a testing house [Go to Page]
Table H.2G.2— Test result of a testing house
H.4.2G.4.2 Test result of a laboratory [Go to Page]
Table H.3G.3— Test result of a laboratory
Annex IH [Go to Page]
(informative) [Go to Page]
I.1H.1 Participants
I.2H.2 Sample [Go to Page]
Table I.1H.1— Sample details for Round robininterlaboratory test
I.4H.4 Test result [Go to Page]
I.4.1H.4.1 Sample A [Go to Page]
Table I.2Table H.2— Round robinInterlaboratory test result for sample A
Table I.3H.3— Round robinInterlaboratory test result for sample B
I.4.3H.4.3 Sample C [Go to Page]
Table I.4H.4 — Round robinInterlaboratory test result for sample C
Table I.5H.5— Summary of Antiviralantiviral activity
Bibliography
30357508_NEW.pdf [Go to Page]
National foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Virus and host cell
6 Warning
7 Apparatus
8 Sterilization of apparatus
9 Reagent and medium [Go to Page]
9.4 Formaldehyde solution.
9.6 Inactivated fetal bovine serum (FBS)
10 Preparation [Go to Page]
10.1 Restoration of host cell from cryopreservation
10.2 Subculture of host cell
10.3 Cell culture for the infectious virus titre assay
10.4 Preparation for test virus [Go to Page]
10.4.1 General
10.4.2 Influenza virus
10.4.3 Feline calicivirus
10.4.4 Infectivity titre of the test viruses
10.5 Preparation for test specimen [Go to Page]
10.5.1 Control fabric
10.5.2 Preparation of test specimens
10.5.3 Sterilization of specimens
10.6 Control test [Go to Page]
10.6.1 General
10.6.2 Verification of cytotoxicity by cell sensitivity to virus and the inactivation of antiviral activity
11 Test procedure [Go to Page]
11.1 Preparation of specimen
11.2 Deposit of virus to the specimens
11.3 Contacting time
11.4 Wash-out of virus immediately after deposit
11.5 Wash-out of virus after contacting time
12 Preparation of the series of the dilution for the virus suspension
13 Determination of the infectious titre [Go to Page]
13.1 Plaque assay
13.2 TCID50 method
14 Calculation of infectivity titre [Go to Page]
14.1 Plaque assay
14.2 TCID50 method [Go to Page]
14.2.1 Behrens and Karber method
14.2.2 Example of calculation
14.3 Test result [Go to Page]
14.3.1 Verification of this test
14.3.2 Calculation of antiviral activity value
15 Test report
Annex A (informative) Virus strains and host cells
Annex B (normative) Infectivity titre test: Plaque assay
Annex C (normative) Infectivity titre test: TCID50 method
Annex D (informative) Composition of media
Annex E (informative) Testing method using specific pathogen free (SPF) embryonated hen's eggs
Annex F (informative) Antiviral efficacy — Antiviral performance of the products
Annex G (informative) Interlaboratory test result (1)
Annex H (informative) Interlaboratory test result (2)
Bibliography [Go to Page]

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BS ISO 18184:2019 - TC Tracked Changes. Textiles. Determination of antiviral activity of textile products, 2020

⨯To continue with accurate pricing,
please select your company type below: