ASTM D5392-93(2000)
Historical Standard: ASTM D5392-93(2000) Standard Test Method for Isolation and Enumeration of Escherichia Coli in Water by the Two-Step Membrane Filter Procedure
SUPERSEDED (see Active link, below)
ASTM D5392
1. Scope
1.1 This test method describes a membrane filter (MF) procedure for the detection and enumeration of Escherichia coli , a bacterium found exclusively in the feces of humans and other warm-blooded animals. The presence of these microorganisms in water is an indication of fecal pollution and the possible presence of enteric pathogens. These bacteria are found in water and wastewater in a wide range of densities. The detection limit of this procedure is one colony forming unit (CFU) per volume filtered.
1.2 This test method has been used successfully with temperate fresh and marine ambient waters, and wastewaters. It is the user's responsibility to ensure the validity of this test method for waters of other types.
1.3 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
ASTM Standards
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
D3370 Practices for Sampling Water from Closed Conduits
D3870 Practice for Establishing Performance Characteristics for Colony Counting Methods in Microbiology
D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
Keywords
ambient waters; Escherichia coli ; fecal pollution; mTEC agar; recreational waters; two-step membrane filter method; wastewaters;
ICS Code
ICS Number Code 07.100.20 (Microbiology of water)
DOI: 10.1520/D5392-93R00
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