ASTM E2563-07
Historical Standard: ASTM E2563-07 Standard Test Method for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method
SUPERSEDED (see Active link, below)
ASTM E2563
1. Scope
1.1 This test method covers the detection and enumeration of viable and culturable rapidly growing Mycobacteria (RGM), or non-tuberculosis Mycobacteria (NTM) in aqueous metalworking fluids (MWF) in the presence of high non-mycobacterial background population using standard microbiological culture methods.
1.2 The detection limit is one colony forming unit (CFU)/mL metalworking fluid.
1.3 This test method involves culture of organisms classified as Level 2 pathogens, and should be undertaken by a trained microbiologist in an appropriately equipped facility. The microbiologist should also be capable of distinguishing the diverse colonies of Mycobacteria from other microorganism colonies on a Petri dish and capable of confirming Mycobacteria by acid fast staining method
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
ASTM Standards
D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
E1326 Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
Keywords
acid-fast bacteria; hypersensitivity pneumonitis; metalworking fluid; non-tuberculous Mycobacteria ; rapidly growing Mycobacteria ;
ICS Code
ICS Number Code 75.100 (Lubricants, industrial oils and related products)
DOI: 10.1520/E2563-07
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