ASTM E2647-08
Historical Standard: ASTM E2647-08 Standard Test Method for Quantification of a Pseudomonas aeruginosa Biofilm Grown Using a Drip Flow Biofilm Reactor with Low Shear and Continuous Flow
SUPERSEDED (see Active link, below)
ASTM E2647
1. Scope
1.1 This test method specifies the operational parameters required to grow a repeatable Pseudomonas aeruginosa biofilm close to the air/liquid interface in a reactor with a continuous flow of nutrients under low fluid shear conditions. The resulting biofilm is representative of generalized situations where biofilm exists at the air/liquid interface under low fluid shear rather than representative of one particular environment.
1.2 This test method uses the drip flow biofilm reactor. The drip flow reactor (DFR) is a plug flow reactor with laminar flow resulting in low fluid shear. The reactor is versatile and may also be used for growing and/or characterizing different species of biofilms.
1.3 This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log colony forming units per surface area.
1.4 Basic microbiology training is required to perform this test method.
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
Other Standard
Method9050 C.1.a Buffered Dilution Water Preparation Eaton, A. D., Clesceri, L. S., and Greenberg, A. E., Eds., Standard Methods for the Examination of Water and Waste Water, 19th Edition, American Public Health Association, American Water Works Association, Water Environment Federation, Washington, DC, 1995.ASTM Standards
D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
Keywords
biofilm; coupons; growth reactor; Pseudomonas aeruginosa ; reactors; sampling; shear;
ICS Code
ICS Number Code 13.060.30 (Sewage water)
DOI: 10.1520/E2647-08
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