1. Scope

1.1 The method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in water-miscible metalworking fluids (MWF).

1.2 The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the samples. The light is produced and measured quantitatively as relative light units (RLU) which are converted by comparison with an ATP standard and computation to pg ATP/mL.

1.3 This method is equally suitable for use in the laboratory or field.

1.4 The method detects ATP concentrations in the range of 4.0 pg ATP/mL to 400,000 pg ATP/mL.

1.5 Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. The method does not differentiate between ATP from different sources, for example, from different types of microorganisms, such as bacteria and fungi.

1.6 The values stated in SI are to be regarded as standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM Standards

D1129 Terminology Relating to Water

D4012 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water

D4840 Guide for Sample Chain-of-Custody Procedures

D6161 Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration and Reverse Osmosis Membrane Processes

E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods

E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method

E1326 Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria

E1497 Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids

E2523 Terminology for Metalworking Fluids and Operations

Keywords

adenosine triphosphate; ATP; bacteria; bioburden; biodeterioration; biomass; fungi; metalworking; microbial contamination; microbiology; microorganisms: Adenosine triphosphate (ATP) content; Biodeterioration; Biomass; Metalworking fluids/related media; Microbial contamination; Microorganisms;

ICS Code

ICS Number Code 75.100 (Lubricants, industrial oils and related products)

DOI: 10.1520/E2694-09

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