ASTM F2260-03(2008)
Historical Standard: ASTM F2260-03(2008) Standard Test Method for Determining Degree of Deacetylation in Chitosan Salts by Proton Nuclear Magnetic Resonance ( 1 H NMR) Spectroscopy
SUPERSEDED (see Active link, below)
ASTM F2260
1. Scope
1.1 This test method covers the determination of the degree of deacetylation in chitosan and chitosan salts intended for use in biomedical and pharmaceutical applications as well as in Tissue Engineered Medical Products (TEMPs) by high-resolution proton NMR ( 1 H NMR). A guide for the characterization of chitosan salts has been published as Guide F 2103 .
1.2 The test method is applicable for determining the degree of deacetylation (% DA) of chitosan chloride and chitosan glutamate salts and is valid for % DA values from 50 up to and including 99. It is simple, rapid, and suitable for routine use. Knowledge of the degree of deacetylation is important for an understanding of the functionality of chitosan salts in TEMP formulations and applications. This test method will assist end users in choosing the correct chitosan for their particular application. Chitosan salts may have utility in drug delivery applications, as a scaffold or matrix material, and in cell and tissue encapsulation applications.
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
European Pharmacopoeia Document
EuropeanPharmacopoei Chitosan Chloride Available from European Directorate for the Quality of Medicines (EDQM), Publications and Services, European Pharmacopoeia, BP 907, F-67029 Strasbourg, France.United States Pharmacopeia Document
USP35-NF30<761> Nuclear Magnetic Resonance Available from U.S. Pharmacopeia (USP), 12601 Twinbrook Pkwy., Rockville, MD 20852-1790, http://www.usp.org.ASTM Standards
F386 Test Method for Thickness of Resilient Flooring Materials Having Flat Surfaces
F2103 Guide for Characterization and Testing of Chitosan Salts as Starting Materials Intended for Use in Biomedical and Tissue-Engineered Medical Product Applications
Keywords
dacetylation; chitosan salts; proton; nuclear magnetic resonance; NMR; spectroscopy;
ICS Code
ICS Number Code 11.120.20 (Wound dressings. Compresses); 71.040.50 (Physicochemical methods of analysis)
DOI: 10.1520/F2260-03R08
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