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  • BSI
    BS EN ISO 16649-3:2015 Microbiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide
    Edition: 2017
    $313.97
    / user per year

Description of BS EN ISO 16649-3:2015 2017

This part of ISO 16649 specifies a horizontal method for the detection and enumeration of β-glucuronidase positive Escherichia coli , by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C. This part of ISO 16649 is applicable to the following:

  • products intended for human consumption and the feeding of animals;

  • environmental samples in the area of food production and food handling.

The method is suitable for the enumeration of cells of E. coli that might have been subjected to stress arising from dehydration, freezing, and exposure to a saline (such as marine) environment or damage by disinfectants such as chlorine-containing products.

A limitation of the applicability of this part of ISO 16649 is imposed by the susceptibility of the method to a large degree of variability. The method is intended to be applied and the results interpreted in the light of the information given in Clause 11.

This method has not been fully evaluated for all matrices (e.g. for milk and milk products). ISO 7251 is intended to be used for milk and milk products.



Standard NumberBS EN ISO 16649-3:2015
TitleMicrobiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide
StatusDefinitive
Publication Date2017-02-28
ReplacesDD ISO/TS 16649-3:2005
CorrectsBS EN ISO 16649-3:2015
DescriptorsTest specimens, Count methods (microbiology), Bacteria count methods, Coliform bacteria, Escherichia coli, Microbiology, Microbiological analysis, Food products, Animal feed, Food testing, Food fit for human consumption, Culture techniques, Biological analysis and testing
ICS07.100.30 Food microbiology
CommitteeAW/9
ISBN978 0 580 97525 7
PublisherBSI
Pages22

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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