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  • BSI
    BS ISO 16649-1:2018 Microbiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Colony-count technique at 44 ° C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide
    Edition: 2018
    $313.97
    / user per year

Description of BS ISO 16649-1:2018 2018

This document specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase[9][10][13][14][17][18][19][20]. It is applicable to

  • products intended for human consumption,

  • products intended for feeding animals,

  • environmental samples in the area of food production and food handling, and

  • samples from the primary production stage such as animal faeces, dust, and swabs.

WARNING Some strains of Escherichia coli may grow poorly or not at all in media incubated at 44 °C. This includes strains of E. coli O157:H7 and O157:H-. Additionally, some strains of Escherichia coli, notably those belonging to serotype O157:H7, are mostly β-glucuronidase negative[11]. Consequently, some strains of E. coli, including pathogenic ones, will not be detected by this method. β-glucuronidase activity may also be exhibited at 44 °C by certain other members of the Enterobacteriaceae, notably Shigella[15] and Salmonella[16].



Standard NumberBS ISO 16649-1:2018
TitleMicrobiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Colony-count technique at 44 ° C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide
StatusConfirmed
Publication Date2018-04-27
ReplacesBS ISO 16649-1:2001
Identical National Standard OfISO 16649-1:2018
DescriptorsFood testing, Coliform bacteria, Microbiological analysis, Membranes, Animal feed, Count methods (microbiology), Escherichia coli, Food products
ICS07.100.30 Food microbiology
CommitteeAW/9
ISBN978 0 580 94562 5
PublisherBSI
Pages20

The following editions for this book are also available...

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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