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  • BSI
    PD ISO/TS 12869:2019 Water quality. Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic amplification by quantitative polymerase chain reaction (qPCR)
    Edition: 2019
    $497.46
    / user per year

Description of PD ISO/TS 12869:2019 2019

This document specifies a method for the detection and quantification of Legionella spp. and L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements.

Technical details specified in this document are given for information only. Any other technical solutions complying with the performance requirements are suitable.

NOTE 1 For performance requirements, see Clause 9.

This document is intended to be applied in the bacteriological investigation of all types of water (hot or cold water, cooling tower water, etc.), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit.

NOTE 2 For validation requirements, see 9.7.

The results are expressed as the number of genome units of Legionella spp. and/or L. pneumophila per litre of sample.

The method described in this document is applicable to all types of water. However, some additives, such as chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.

The qPCR methods do not give any information about the physiological state of the Legionella.



Standard NumberPD ISO/TS 12869:2019
TitleWater quality. Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic amplification by quantitative polymerase chain reaction (qPCR)
StatusConfirmed
Publication Date2019-04-09
ReplacesPD ISO/TS 12869:2012
Identical National Standard OfISO/TS 12869
DescriptorsBacterial viruses, Quality assurance, Turbidity, Biological analysis and testing, Quality control, Sampling methods, Calibration, Test equipment, Bacteria count methods, Dilution, Water pollution, Viruses, Water testing, Microbiological analysis, Testing conditions, Quality, Count methods (microbiology), Biological water pollutants, Reagent solutions, Water, Culture media, Biological hazards, Safety measures
ICS07.100.20 Microbiology of water
CommitteeEH/3/4
ISBN978 0 580 98895 0
PublisherBSI
Pages56

The following editions for this book are also available...

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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