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16/30337114 DC BS EN 16987. Foodstuffs. Determination of acrylamide in coffee and coffee products by HPLC-MS/MS and GC-MS >
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16/30337114 DC BS EN 16987. Foodstuffs. Determination of acrylamide in coffee and coffee products by HPLC-MS/MS and GC-MS, 2016
- Contents Page
- European foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Principle
- 4 Reagents
- 5 Apparatus
- 6 Procedure [Go to Page]
- Table 1 — Mass spectrometric transitions used for the identification and quantification of acrylamide [Go to Page]
- Table 2 — Observed ions
- 7 Calibration [Go to Page]
- 8 Evaluation [Go to Page]
- 9 Precision data [Go to Page]
- Table 3 — Precision data for the determination of acrylamide in coffee [Go to Page]
- 10 Measurement uncertainty
- 11 Test report
- Annex A (informative) Performance characteristics
- Table A.1 — Inter-laboratory study results (for both HPLC-and GC variants)0F )
- Annex B (informative) Examples of absorber materials
- Table B.1 — Examples of suitable sorbents for the SPE clean-up (HPLC and GC, see 6.3)
- Table B.2 — Examples of suitable sorbents for the SPE clean-up of the GC extract (see 6.5.1)
- Annex C (informative) Examples for recommended columns and analysis conditions
- C.1 Generally suitable chromatographic columns
- Table C.1 — Analytical conditions for selected HPLC columns
- Table C.2 — GC capillary columns
- C.2 Example method and approximate retention time for HPLC-MS/MS
- C.2.1 Example A
- Table C.3 — Chromatographic conditions for example A
- C.2.2 Example B
- Table C.4 — Chromatographic conditions for example B
- C.3 Example of suitable conditions and approximate retention time for GC-MS analysis
- Table C.5 — GC method: Example of suitable conditions and approximate retention time for GC-MS
- C.4 Example chromatograms for HPLC-MS/MS
- Figure C.1 — D3-acrylamide
- Figure C.2 — Acrylamide, native
- C.5 Example of chromatogram for GC-MS
- Figure C.3 — Total ion chromatogram (scan mode)
- Figure C.4 — Extracted ion, mass 149 (quantifier ion)
- Figure C.5 — Extracted ion, mass 106 (qualifier ion)
- Bibliography [Go to Page]