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BS EN ISO 11290-1:2017 Microbiology of the food chain. Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Detection method >
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BS EN ISO 11290-1:2017 Microbiology of the food chain. Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Detection method, 2017
- National foreword
- European foreword
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 4.1 General
- 4.2 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half-Fraser broth)
- 4.3 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
- 4.4 Plating out and identification
- 4.5 Confirmation
- 5 Culture media and reagents
- 6 Equipment and consumables
- 7 Sampling
- 8 Preparation of test sample
- 9 Procedure
- 9.1 Test portion and initial suspension
- 9.2 Primary enrichment
- 9.3 Secondary enrichment
- 9.4 Plating out and identification
- 9.4.1 General
- 9.4.2 Agar Listeria according to Ottaviani and Agosti
- 9.4.3 Second selective medium
- 9.5 Confirmation of Listeria monocytogenes or Listeria spp.
- 9.5.1 Selection of colonies for confirmation
- 9.5.2 Confirmation of L. monocytogenes
- 9.5.3 Confirmation of Listeria spp.
- 9.6 Interpretation of morphological and physiological properties and of the biochemical reactions
- 9.7 Additional characterization of isolated strains (optional)
- 10 Expression of results
- 11 Performance characteristics of the method
- 11.1 Method validation studies
- 11.2 Sensitivity
- 11.3 Specificity
- 11.4 Level of detection (LOD50)
- 12 Test report
- 13 Quality assurance
- Annex A (normative) Diagram of procedure
- Annex B (normative) Composition and preparation of culture media and reagents
- Annex C (informative) Distinction of Listeria spp. from other genera
- Annex D (informative) Reactions for the identification of Listeria species
- Annex E (informative) Listeria selective agars
- Annex F (informative) Results of the interlaboratory studies for detection of Listeria monocytogenes
- Bibliography [Go to Page]